Fig. 2. Effect of AVP and apelin-13 treatment on intracellular cAMP levels. mpkCCD cells grown on permeable supports were treated with HBSS, 1 nM of AVP, or with a combination of 1nM AVP and 200 nM of apelin-13 for 1 hour at 37˚C. Each data set represents the mean of three independent experiments, each performed in triplicate, and expressed as the mean ± S.E.M. *p<0.05 one-way ANOVA with a post-hoc Bonferroni multiple comparison test.